Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.
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ABSTRACT:
SUBMITTER: Bryksin AV
PROVIDER: S-EPMC2951906 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Publications
Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.
Bryksin Anton V AV Matsumura Ichiro I
PloS one 20101008 10
<h4>Background</h4>Most plasmids replicate only within a particular genus or family.<h4>Methodology/principal findings</h4>Here we describe an engineered high copy number expression vector, pBAV1K-T5, that produces varying quantities of active reporter proteins in Escherichia coli, Acinetobacter baylyi ADP1, Agrobacterium tumefaciens, (all gram-negative), Streptococcus pneumoniae, Leifsonia shinshuensis, Peanibacillus sp. S18-36 and Bacillus subtilis (gram-positive).<h4>Conclusions/significance< ...[more]
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