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Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.


ABSTRACT: Most plasmids replicate only within a particular genus or family.Here we describe an engineered high copy number expression vector, pBAV1K-T5, that produces varying quantities of active reporter proteins in Escherichia coli, Acinetobacter baylyi ADP1, Agrobacterium tumefaciens, (all gram-negative), Streptococcus pneumoniae, Leifsonia shinshuensis, Peanibacillus sp. S18-36 and Bacillus subtilis (gram-positive).Our results demonstrate the efficiency of pBAV1K-T5 replication in different bacterial species, thereby facilitating the study of proteins that don't fold well in E. coli and pathogens not amenable to existing genetic tools.

SUBMITTER: Bryksin AV 

PROVIDER: S-EPMC2951906 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.

Bryksin Anton V AV   Matsumura Ichiro I  

PloS one 20101008 10


<h4>Background</h4>Most plasmids replicate only within a particular genus or family.<h4>Methodology/principal findings</h4>Here we describe an engineered high copy number expression vector, pBAV1K-T5, that produces varying quantities of active reporter proteins in Escherichia coli, Acinetobacter baylyi ADP1, Agrobacterium tumefaciens, (all gram-negative), Streptococcus pneumoniae, Leifsonia shinshuensis, Peanibacillus sp. S18-36 and Bacillus subtilis (gram-positive).<h4>Conclusions/significance<  ...[more]

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