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Engineered lactic acid bacterium Lactococcus lactis capable of binding antibodies and tumor necrosis factor alpha.

ABSTRACT: We have optimized the display of the B domain of staphylococcal protein A on the surface of Lactococcus lactis. The maximum binding capacity was estimated at 0.146 ?g of antibody per 10? cells and was sustained at 86% after treatment with simulated gastric juice. A tumor necrosis factor alpha (TNF-?)-binding affibody was also displayed and bound TNF-?, which could be useful in the treatment of inflammatory bowel disease.

SUBMITTER: Ravnikar M

PROVIDER: S-EPMC2953040 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Engineered lactic acid bacterium Lactococcus lactis capable of binding antibodies and tumor necrosis factor alpha.

Ravnikar Matjaz M Strukelj Borut B Obermajer Natasa N Lunder Mojca M Berlec Ales A

Applied and environmental microbiology 20100827 20

We have optimized the display of the B domain of staphylococcal protein A on the surface of Lactococcus lactis. The maximum binding capacity was estimated at 0.146 μg of antibody per 10⁸ cells and was sustained at 86% after treatment with simulated gastric juice. A tumor necrosis factor alpha (TNF-α)-binding affibody was also displayed and bound TNF-α, which could be useful in the treatment of inflammatory bowel disease. ...[more]

PMID: 20802083

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