Unknown

Dataset Information

0

SANS investigation of the photosynthetic machinery of Chloroflexus aurantiacus.


ABSTRACT: Green photosynthetic bacteria harvest light and perform photosynthesis in low-light environments, and contain specialized antenna complexes to adapt to this condition. We performed small-angle neutron scattering (SANS) studies to obtain structural information about the photosynthetic apparatus, including the peripheral light-harvesting chlorosome complex, the integral membrane light-harvesting B808-866 complex, and the reaction center (RC) in the thermophilic green phototrophic bacterium Chloroflexus aurantiacus. Using contrast variation in SANS measurements, we found that the B808-866 complex is wrapped around the RC in Cfx. aurantiacus, and the overall size and conformation of the B808-866 complex of Cfx. aurantiacus is roughly comparable to the LH1 antenna complex of the purple bacteria. A similar size of the isolated B808-866 complex was suggested by dynamic light scattering measurements, and a smaller size of the RC of Cfx. aurantiacus compared to the RC of the purple bacteria was observed. Further, our SANS measurements indicate that the chlorosome is a lipid body with a rod-like shape, and that the self-assembly of bacteriochlorophylls, the major component of the chlorosome, is lipid-like. Finally, two populations of chlorosome particles are suggested in our SANS measurements.

SUBMITTER: Tang KH 

PROVIDER: S-EPMC2956220 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

SANS investigation of the photosynthetic machinery of Chloroflexus aurantiacus.

Tang Kuo-Hsiang KH   Urban Volker S VS   Wen Jianzhong J   Xin Yueyong Y   Blankenship Robert E RE  

Biophysical journal 20101001 8


Green photosynthetic bacteria harvest light and perform photosynthesis in low-light environments, and contain specialized antenna complexes to adapt to this condition. We performed small-angle neutron scattering (SANS) studies to obtain structural information about the photosynthetic apparatus, including the peripheral light-harvesting chlorosome complex, the integral membrane light-harvesting B808-866 complex, and the reaction center (RC) in the thermophilic green phototrophic bacterium Chlorof  ...[more]

Similar Datasets

| S-EPMC415758 | biostudies-literature
| S-EPMC6959224 | biostudies-literature
| S-EPMC2914828 | biostudies-literature
| S-EPMC7308566 | biostudies-literature
| PRJNA35073 | ENA
| S-EPMC2681904 | biostudies-other
| S-EPMC2795307 | biostudies-literature
| S-EPMC3150298 | biostudies-literature
| S-EPMC4450660 | biostudies-literature
| S-EPMC3182672 | biostudies-literature