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Evaluating genome-scale approaches to eukaryotic DNA replication.

ABSTRACT: Mechanisms regulating where and when eukaryotic DNA replication initiates remain a mystery. Recently, genome-scale methods have been brought to bear on this problem. The identification of replication origins and their associated proteins in yeasts is a well-integrated investigative tool, but corresponding data sets from multicellular organisms are scarce. By contrast, standardized protocols for evaluating replication timing have generated informative data sets for most eukaryotic systems. Here, I summarize the genome-scale methods that are most frequently used to analyse replication in eukaryotes, the kinds of questions each method can address and the technical hurdles that must be overcome to gain a complete understanding of the nature of eukaryotic replication origins.

SUBMITTER: Gilbert DM

PROVIDER: S-EPMC2962615 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Evaluating genome-scale approaches to eukaryotic DNA replication.

Gilbert David M DM

Nature reviews. Genetics 20100901 10

Mechanisms regulating where and when eukaryotic DNA replication initiates remain a mystery. Recently, genome-scale methods have been brought to bear on this problem. The identification of replication origins and their associated proteins in yeasts is a well-integrated investigative tool, but corresponding data sets from multicellular organisms are scarce. By contrast, standardized protocols for evaluating replication timing have generated informative data sets for most eukaryotic systems. Here, ...[more]

PMID: 20811343

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