Unknown

Dataset Information

0

Factors from human embryonic stem cell-derived fibroblast-like cells promote topology-dependent hepatic differentiation in primate embryonic and induced pluripotent stem cells.


ABSTRACT: The future clinical use of embryonic stem cell (ESC)-based hepatocyte replacement therapy depends on the development of an efficient procedure for differentiation of hepatocytes from ESCs. Here we report that a high density of human ESC-derived fibroblast-like cells (hESdFs) supported the efficient generation of hepatocyte-like cells with functional and mature hepatic phenotypes from primate ESCs and human induced pluripotent stem cells. Molecular and immunocytochemistry analyses revealed that hESdFs caused a rapid loss of pluripotency and induced a sequential endoderm-to-hepatocyte differentiation in the central area of ESC colonies. Knockdown experiments demonstrated that pluripotent stem cells were directed toward endodermal and hepatic lineages by FGF2 and activin A secreted from hESdFs. Furthermore, we found that the central region of ESC colonies was essential for the hepatic endoderm-specific differentiation, because its removal caused a complete disruption of endodermal differentiation. In conclusion, we describe a novel in vitro differentiation model and show that hESdF-secreted factors act in concert with regional features of ESC colonies to induce robust hepatic endoderm differentiation in primate pluripotent stem cells.

SUBMITTER: Huang HP 

PROVIDER: S-EPMC2963358 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Factors from human embryonic stem cell-derived fibroblast-like cells promote topology-dependent hepatic differentiation in primate embryonic and induced pluripotent stem cells.

Huang Hsiang-Po HP   Yu Chun-Ying CY   Chen Hsin-Fu HF   Chen Pin-Hsun PH   Chuang Ching-Yu CY   Lin Sung-Jan SJ   Huang Shih-Tsung ST   Chan Wei-Hung WH   Ueng Tzuu-Huei TH   Ho Hong-Nerng HN   Kuo Hung-Chih HC  

The Journal of biological chemistry 20100818 43


The future clinical use of embryonic stem cell (ESC)-based hepatocyte replacement therapy depends on the development of an efficient procedure for differentiation of hepatocytes from ESCs. Here we report that a high density of human ESC-derived fibroblast-like cells (hESdFs) supported the efficient generation of hepatocyte-like cells with functional and mature hepatic phenotypes from primate ESCs and human induced pluripotent stem cells. Molecular and immunocytochemistry analyses revealed that h  ...[more]

Similar Datasets

2010-08-20 | E-GEOD-19964 | biostudies-arrayexpress
2010-08-20 | GSE19964 | GEO
| S-EPMC3011000 | biostudies-literature
| S-EPMC5738144 | biostudies-literature
| S-EPMC7041531 | biostudies-literature
| S-EPMC3105203 | biostudies-literature
| S-EPMC4672282 | biostudies-literature
| S-EPMC3411998 | biostudies-literature
| S-EPMC3403099 | biostudies-literature
| S-EPMC3822923 | biostudies-literature