Ontology highlight
ABSTRACT:
SUBMITTER: Baak-Pablo R
PROVIDER: S-EPMC2963916 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Baak-Pablo Renee R Dezentje Vincent V Guchelaar Henk-Jan HJ van der Straaten Tahar T
The Journal of molecular diagnostics : JMD 20100916 6
DNA isolated from formalin-fixed paraffin-embedded (FFPE) tissue is often fragmented and cross-linked and is therefore difficult to genotype. To enable this source of DNA for genotyping analysis using Taqman probes, we tested whether enrichment of the target genes would increase the amount of available DNA. For enrichment of the target genes, we used preamplification by means of diluted Taqman assays. To establish the appropriateness of preamplification, we used DNA extracted from paraffin-embed ...[more]