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Cytochrome c oxidase loses catalytic activity and structural integrity during the aging process in Drosophila melanogaster.

ABSTRACT: The hypothesis, that structural deterioration of cytochrome c oxidase (CcO) is a causal factor in the age-related decline in mitochondrial respiratory activity and an increase in H?O? generation, was tested in Drosophila melanogaster. CcO activity and the levels of seven different nuclear DNA-encoded CcO subunits were determined at three different stages of adult life, namely, young-, middle-, and old-age. CcO activity declined progressively with age by 33%. Western blot analysis, using antibodies specific to Drosophila CcO subunits IV, Va, Vb, VIb, VIc, VIIc, and VIII, indicated that the abundance these polypeptides decreased, ranging from 11% to 40%, during aging. These and previous results suggest that CcO is a specific intra-mitochondrial site of age-related deterioration, which may have a broad impact on mitochondrial physiology.

SUBMITTER: Ren JC

PROVIDER: S-EPMC2964050 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Cytochrome c oxidase loses catalytic activity and structural integrity during the aging process in Drosophila melanogaster.

Ren Jian-Ching JC Rebrin Igor I Klichko Vladimir V Orr William C WC Sohal Rajindar S RS

Biochemical and biophysical research communications 20100915 1

The hypothesis, that structural deterioration of cytochrome c oxidase (CcO) is a causal factor in the age-related decline in mitochondrial respiratory activity and an increase in H₂O₂ generation, was tested in Drosophila melanogaster. CcO activity and the levels of seven different nuclear DNA-encoded CcO subunits were determined at three different stages of adult life, namely, young-, middle-, and old-age. CcO activity declined progressively with age by 33%. Western blot analysis, using antibodi ...[more]

PMID: 20833144

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