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Fluorescent Gold Nanoprobes for the Sensitive and Selective Detection for Hg.

ABSTRACT: A simple, cost-effective yet rapid and sensitive sensor for on-site and real-time Hg(2+) detection based on bovine serum albumin functionalized fluorescent gold nanoparticles as novel and environmentally friendly fluorescent probes was developed. Using this probe, aqueous Hg(2+) can be detected at 0.1 nM in a facile way based on fluorescence quenching. This probe was also applied to determine the Hg(2+) in the lake samples, and the results demonstrate low interference and high sensitivity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11671-010-9730-y) contains supplementary material, which is available to authorized users.

SUBMITTER: Chai F

PROVIDER: S-EPMC2964487 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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Fluorescent Gold Nanoprobes for the Sensitive and Selective Detection for Hg.

Chai Fang F Wang Tingting T Li Lu L Liu Haiyan H Zhang Lingyu L Su Zhongmin Z Wang Chungang C

Nanoscale research letters 20100902 11

A simple, cost-effective yet rapid and sensitive sensor for on-site and real-time Hg(2+) detection based on bovine serum albumin functionalized fluorescent gold nanoparticles as novel and environmentally friendly fluorescent probes was developed. Using this probe, aqueous Hg(2+) can be detected at 0.1 nM in a facile way based on fluorescence quenching. This probe was also applied to determine the Hg(2+) in the lake samples, and the results demonstrate low interference and high sensitivity. ELECT ...[more]

PMID: 21124635

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Project description:Arsenic is considered as a toxic heavy metal which is highly detrimental to ecological systems, and long-term exposure to it is highly dangerous to life as it can cause serious health effects. Timely detection of traces of active arsenic (As3+) is very crucial, and the development of simple, cost-effective methods is imperative to address the presence of arsenic in water and food chain. Herein, we present an extensive study on chemical-free electrogenerated nanotextured gold assemblage for the detection of ultralow levels of As3+ in water up to 0.08 ppb concentration. The gold nanotextured electrode (Au/GNE) is developed on simple Au foil via electrochemical oxidation-reduction sweeps in a metal-ion-free electrolyte solution. The ultrafine nanoscale morphological attributes of Au/GNE substrate are studied by scanning electron microscopy. Square wave anodic stripping voltammetry (ASV) response for different concentrations of arsenites is determined and directly correlated with As3+ detection regarding the type of electrolyte solution, deposition potential, and deposition time. The average of three standard curves are linear from 0.1 ppb up to 9 ppb (n = 15) with a linear regression coefficient R 2 = 0.9932. Under optimized conditions, a superior sensitivity of 39.54 ?A ppb-1 cm-2 is observed with a lower detection limit of 0.1 ppb (1.3 nM) (based on the visual analysis of calibration curve) and 0.08 ppb (1.06 nM) (based on the standard deviation of linear regression). Furthermore, the electrochemical Au/GNE is also applicable for arsenic detection in a complex system containing Cu2+, Ni2+, Fe2+, Pb2+, Hg2+, and other ions for the selective and sensitive analysis. Au/GNE substrate also possesses remarkable reproducibility and high stability for arsenic detection during repeated analysis and thus can be employed for prolonged applications and reiterating analyses. This electrochemically generated nanotextured electrode is also applicable for As3+ detection and analysis in a real water sample under optimized conditions. Therefore, fabrication conditions and analytical and electroanalytical performances justify that because of its low cost, easy preparation method and assembly, high reproducibility, and robustness, nanosensor Au/GNE can be scaled up for further applications.

Project description:BACKGROUND: In situation like diagnosis of clinical and forensic samples there exists a need for highly sensitive, rapid and specific DNA detection methods. Though conventional DNA amplification using PCR can provide fast results, it is not widely practised in diagnostic laboratories partially because it requires skilled personnel and expensive equipment. To overcome these limitations nanoparticles have been explored as signalling probes for ultrasensitive DNA detection that can be used in field applications. Among the nanomaterials, gold nanoparticles (AuNPs) have been extensively used mainly because of its optical property and ability to get functionalized with a variety of biomolecules. RESULTS: We report a protocol for the use of gold nanoparticles functionalized with single stranded oligonucleotide (AuNP- oligo probe) as visual detection probes for rapid and specific detection of Escherichia coli. The AuNP- oligo probe on hybridization with target DNA containing complementary sequences remains red whereas test samples without complementary DNA sequences to the probe turns purple due to acid induced aggregation of AuNP- oligo probes. The color change of the solution is observed visually by naked eye demonstrating direct and rapid detection of the pathogenic Escherichia coli from its genomic DNA without the need for PCR amplification. The limit of detection was ~54 ng for unamplified genomic DNA. The method requires less than 30 minutes to complete after genomic DNA extraction. However, by using unamplified enzymatic digested genomic DNA, the detection limit of 11.4 ng was attained. Results of UV-Vis spectroscopic measurement and AFM imaging further support the hypothesis of aggregation based visual discrimination. To elucidate its utility in medical diagnostic, the assay was validated on clinical strains of pathogenic Escherichia coli obtained from local hospitals and spiked urine samples. It was found to be 100% sensitive and proves to be highly specific without any cross reaction with non-Escherichia coli strains. CONCLUSION: This work gives entry into a new class of DNA/gold nanoparticles hybrid materials which might have optical property that can be controlled for application in diagnostics. We note that it should be possible to extend this strategy easily for developing new types of DNA biosensor for point of care detection. The salient feature of this approach includes low-cost, robust reagents and simple colorimetric detection of pathogen.

Dataset Information

Fluorescent Gold Nanoprobes for the Sensitive and Selective Detection for Hg.

Publications

Fluorescent Gold Nanoprobes for the Sensitive and Selective Detection for Hg.

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