Ontology highlight
ABSTRACT:
SUBMITTER: Monsen VT
PROVIDER: S-EPMC2965238 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Monsen Vivi Talstad VT Sundheim Ottar O Aas Per Arne PA Westbye Marianne P MP Sousa Mirta M L MM Slupphaug Geir G Krokan Hans E HE
Nucleic acids research 20100604 19
Human AlkB homologues ABH2 and ABH3 repair 1-methyladenine and 3-methylcytosine in DNA/RNA by oxidative demethylation. The enzymes have similar overall folds and active sites, but are functionally divergent. ABH2 efficiently demethylates both single- and double-stranded (ds) DNA, whereas ABH3 has a strong preference for single-stranded DNA and RNA. We find that divergent F1 β-hairpins in proximity of the active sites of ABH2 and ABH3 are central for substrate specificities. Swapping F1 hairpins ...[more]