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Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA.

ABSTRACT: The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes.

SUBMITTER: Szwagierczak A

PROVIDER: S-EPMC2965258 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA.

Szwagierczak Aleksandra A Bultmann Sebastian S Schmidt Christine S CS Spada Fabio F Leonhardt Heinrich H

Nucleic acids research 20100804 19

The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression ...[more]

PMID: 20685817

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High-resolution enzymatic mapping of genomic 5-hydroxymethylcytosine in mouse embryonic stem cells.

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High-resolution enzymatic mapping of genomic 5-hydroxymethylcytosine in mouse embryonic stem cells

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High-resolution enzymatic mapping of genomic 5-hydroxymethylcytosine in mouse embryonic stem cells

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