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Allosteric inhibition of the epithelial Na+ channel through peptide binding at peripheral finger and thumb domains.

ABSTRACT: The epithelial Na(+) channel (ENaC) mediates the rate-limiting step in transepithelial Na(+) transport in the distal segments of the nephron and in the lung. ENaC subunits are cleaved by proteases, resulting in channel activation due to the release of inhibitory tracts. Peptides derived from these tracts inhibit channel activity. The mechanism by which these intrinsic inhibitory tracts reduce channel activity is unknown, as are the sites where these tracts interact with other residues within the channel. We performed site-directed mutagenesis in large portions of the predicted periphery of the extracellular region of the ? subunit and measured the effect of mutations on an 8-residue inhibitory tract-derived peptide. Our data show that the inhibitory peptide likely binds to specific residues within the finger and thumb domains of ENaC. Pairwise interactions between the peptide and the channel were identified by double mutant cycle experiments. Our data suggest that the inhibitory peptide has a specific peptide orientation within its binding site. Extended to the intrinsic inhibitory tract, our data suggest that proteases activate ENaC by removing residues that bind at the finger-thumb domain interface.

SUBMITTER: Kashlan OB

PROVIDER: S-EPMC2966135 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

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Allosteric inhibition of the epithelial Na+ channel through peptide binding at peripheral finger and thumb domains.

Kashlan Ossama B OB Boyd Cary R CR Argyropoulos Christos C Okumura Sora S Hughey Rebecca P RP Grabe Michael M Kleyman Thomas R TR

The Journal of biological chemistry 20100903 45

The epithelial Na(+) channel (ENaC) mediates the rate-limiting step in transepithelial Na(+) transport in the distal segments of the nephron and in the lung. ENaC subunits are cleaved by proteases, resulting in channel activation due to the release of inhibitory tracts. Peptides derived from these tracts inhibit channel activity. The mechanism by which these intrinsic inhibitory tracts reduce channel activity is unknown, as are the sites where these tracts interact with other residues within the ...[more]

PMID: 20817728

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Project description:Mammalian ASIC channels of the DEG/ENaC superfamily are gated by extracellular protons and function to mediate touch and pain sensitivity, learning and memory, and fear conditioning. The recently solved crystal structure of chicken ASIC1a and preliminary functional studies suggested that a highly negatively charged pocket in the extracellular domain of the channel might be the primary proton binding domain. However, more recent extensive mutagenesis analysis paints a more complex mechanism of channel gating, involving binding of protons at sites immediately after the first transmembrane domain (TM1) and displacement of inhibitory Ca(2+) ions from the acidic pocket in the extracellular domain and from another Ca(2+) binding site at the mouth of the pore. We recently identified and functionally characterized Caenorhabditis elegans ACD-1, the first acid-inactivated DEG/ENaC channel. ACD-1 is expressed in C. elegans amphid glia and functions with neuronal DEG/ENaC channel DEG-1 to mediate acid avoidance and chemotaxis to the amino acid lysine. The post-TM1 residues that were proposed to bind protons in ASIC1a are not conserved in ACD-1, but some of the amino acids constituting the acidic pocket are. However, ACD-1 proton sensitivity is completely independent from extracellular Ca(2+), and protons appear to bind the channel in a less cooperative manner. We thus wondered if residues in the acidic pocket might contribute to ACD-1 acid sensitivity. We show here that while ACD-1 sensitivity to extracellular protons is influenced by mutations in the acidic pocket, other sites are likely to participate. We also report that one histidine at the base of the thumb and residues in the channel pore influence proton inhibition in a voltage-independent manner, suggesting that they affect the coupling of proton binding with the gating rather than proton binding itself. We conclude that ACD-1 inhibition by protons is likely mediated by binding of proton ions to multiple sites throughout the extracellular domain of the channel. Our data also support a model in which residues in the acidic pocket contribute to determining the channel state perhaps by changing the strength of the interaction between adjacent thumb and finger domains.

Dataset Information

Allosteric inhibition of the epithelial Na+ channel through peptide binding at peripheral finger and thumb domains.

Publications

Allosteric inhibition of the epithelial Na+ channel through peptide binding at peripheral finger and thumb domains.

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