Ontology highlight
ABSTRACT:
SUBMITTER: Thyagarajan A
PROVIDER: S-EPMC2966532 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Thyagarajan Amar A Ting Alice Y AY
Cell 20101007 3
The functions of trans-synaptic adhesion molecules, such as neurexin and neuroligin, have been difficult to study due to the lack of methods to directly detect their binding in living neurons. Here, we use biotin labeling of intercellular contacts (BLINC), a method for imaging protein interactions based on interaction-dependent biotinylation of a peptide by E. coli biotin ligase, to visualize neurexin-neuroligin trans-interactions at synapses and study their role in synapse development. We found ...[more]