Ontology highlight
ABSTRACT:
SUBMITTER: Chen JC
PROVIDER: S-EPMC2967418 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Chen Julian C H JC Mustyakimov Marat M Schoenborn Benno P BP Langan Paul P Blum Marc Michael MM
Acta crystallographica. Section D, Biological crystallography 20101020 Pt 11
Diisopropyl fluorophosphatase (DFPase) is a calcium-dependent phosphotriesterase that acts on a variety of highly toxic organophosphorus compounds that act as inhibitors of acetylcholinesterase. The mechanism of DFPase has been probed using a variety of methods, including isotopic labelling, which demonstrated the presence of a phosphoenzyme intermediate in the reaction mechanism. In order to further elucidate the mechanism of DFPase and to ascertain the protonation states of the residues and so ...[more]