Fewer essential genes in mycoplasmas than previous studies suggest.
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ABSTRACT:
SUBMITTER: Dybvig K
PROVIDER: S-EPMC2974803 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Publications
Fewer essential genes in mycoplasmas than previous studies suggest.
Dybvig Kevin K Lao Ping P Jordan David S DS Simmons Warren L WL
FEMS microbiology letters 20100816 1
Here, we describe mutants of Mycoplasma pulmonis that were obtained using a minitransposon, Tn4001TF1, which actively transposes but is then unable to undergo subsequent excision events. Using Tn4001TF1, we disrupted 39 genes previously thought to be essential for growth. Thus, the number of genes required for growth has been overestimated. This study also revealed evidence of gene duplications in M. pulmonis and identified chromosome segregation proteins that are dispensable in mycoplasmas but ...[more]
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