Unknown

Dataset Information

0

Amplification-free digital gene expression profiling from minute cell quantities.


ABSTRACT: Generating reliable expression profiles from minute cell quantities is critical for scientific discovery and potential clinical applications. Here we present low-quantity digital gene expression (LQ-DGE), an amplification-free approach involving capture of poly(A)(+) RNAs from cellular lysates onto poly(dT)-coated sequencing surfaces, followed by on-surface reverse transcription and sequencing. We applied LQ-DGE to profile malignant and nonmalignant mouse and human cells, demonstrating its quantitative power and potential applicability to archival specimens.

SUBMITTER: Ozsolak F 

PROVIDER: S-EPMC2975601 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC4165214 | biostudies-literature
| S-EPMC5605290 | biostudies-literature
| S-EPMC2362006 | biostudies-literature
| S-EPMC6287822 | biostudies-literature
| S-EPMC3377998 | biostudies-literature
| S-EPMC5819784 | biostudies-literature
| S-EPMC4348626 | biostudies-literature
| S-EPMC1578511 | biostudies-literature
2018-11-23 | PXD011175 | Pride
| S-EPMC3598852 | biostudies-literature