Ontology highlight
ABSTRACT:
SUBMITTER: Kongmanas K
PROVIDER: S-EPMC2975728 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Kongmanas Kessiri K Xu Hongbin H Yaghoubian Arman A Franchini Laura L Panza Luigi L Ronchetti Fiamma F Faull Kym K Tanphaichitr Nongnuj N
Journal of lipid research 20100903 12
Seminolipid, also known as sulfogalactosylglycerolipid (SGG), plays important roles in male reproduction. Therefore, an accurate and sensitive method for SGG quantification in testes and sperm is needed. Here we compare SGG quantitation by the traditional colorimetric Azure A assay with LC-ESI-MS/MS using multiple reaction monitoring (MRM). Inclusion of deuterated SGG as the internal standard endowed accuracy to the MRM method. The results showed reasonable agreement between the two procedures f ...[more]