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OliI, a unique restriction endonuclease that recognizes the discontinuous sequence 5'-CACNN NGTG-3'.


ABSTRACT: A new type II restriction endonuclease designated OLI:I has been partially purified from the halophilic bacterium Oceanospirillum linum 4-5D. OLI:I recognizes the interrupted hexanucleotide palindrome 5'-CACNN NNGTG-3' and cleaves it in the center generating blunt-ended DNA fragments.

SUBMITTER: Padegimiene E 

PROVIDER: S-EPMC29759 | biostudies-literature | 2001 Mar

REPOSITORIES: biostudies-literature

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OliI, a unique restriction endonuclease that recognizes the discontinuous sequence 5'-CACNN NGTG-3'.

Padegimiene E E   Maneliene Z Z   Petruyte M M   Janulaitis A A  

Nucleic acids research 20010301 6


A new type II restriction endonuclease designated OLI:I has been partially purified from the halophilic bacterium Oceanospirillum linum 4-5D. OLI:I recognizes the interrupted hexanucleotide palindrome 5'-CACNN NNGTG-3' and cleaves it in the center generating blunt-ended DNA fragments. ...[more]

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