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Stable, site-specific fluorescent tagging constructs optimized for burkholderia species.

ABSTRACT: Several vectors that facilitate stable fluorescent labeling of Burkholderia pseudomallei and Burkholderia thailandensis were constructed. These vectors combined the effectiveness of the mini-Tn7 site-specific transposition system with fluorescent proteins optimized for Burkholderia spp., enabling bacterial tracking during cellular infection.

SUBMITTER: Norris MH

PROVIDER: S-EPMC2976199 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

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Stable, site-specific fluorescent tagging constructs optimized for burkholderia species.

Norris Michael H MH Kang Yun Y Wilcox Bruce B Hoang Tung T TT

Applied and environmental microbiology 20100917 22

Several vectors that facilitate stable fluorescent labeling of Burkholderia pseudomallei and Burkholderia thailandensis were constructed. These vectors combined the effectiveness of the mini-Tn7 site-specific transposition system with fluorescent proteins optimized for Burkholderia spp., enabling bacterial tracking during cellular infection. ...[more]

PMID: 20851961

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