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Genetic transformation and mutagenesis via single-stranded DNA in the unicellular, diazotrophic cyanobacteria of the genus Cyanothece.

ABSTRACT: We describe a genetic system for producing specific gene knockouts in Cyanothece sp. strain PCC 7822 using a single-stranded DNA technique (B. Zorin, P. Hegemann, and I. Sizova, Eukaryot. Cell 4:1264-1272, 2005). The first fully segregated mutant was a ?nifK mutant, and it was unable to grow on medium lacking combined nitrogen and produced virtually no hydrogen.

SUBMITTER: Min H 

PROVIDER: S-EPMC2976206 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

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Genetic transformation and mutagenesis via single-stranded DNA in the unicellular, diazotrophic cyanobacteria of the genus Cyanothece.

Min Hongtao H   Sherman Louis A LA  

Applied and environmental microbiology 20100917 22

We describe a genetic system for producing specific gene knockouts in Cyanothece sp. strain PCC 7822 using a single-stranded DNA technique (B. Zorin, P. Hegemann, and I. Sizova, Eukaryot. Cell 4:1264-1272, 2005). The first fully segregated mutant was a ΔnifK mutant, and it was unable to grow on medium lacking combined nitrogen and produced virtually no hydrogen. ...[more]

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