Dataset Information

Isolation of a novel bacteriophage specific for the periodontal pathogen Fusobacterium nucleatum.

ABSTRACT: Fusobacterium nucleatum is a periodontal pathogen that has been directly associated with the development and progression of periodontal disease, a widespread pathology that affects the support tissues of the tooth. We isolated a new bacteriophage (Fnp?02) that specifically infects this bacterium. Transmission electron microscopy showed that the virion is composed of an icosahedral head and a segmented tail. The size of the phage genome was estimated to be approximately 59 kbp of double-stranded DNA. The morphological features and the genetic characteristics suggest that Fnp?02 is part of the Siphoviridae family. Using one-step growth and adsorption experiments, the latent period, burst size, and adsorption rate were estimated to be 15 h, 100 infectious units per cell, and 7.5 × 10?¹? ml min?¹, respectively. A small fragment of phage DNA was cloned and sequenced, showing 93% nucleotide identity with the phage PA6 of Propionibacterium acnes and amino acid identity with fragments of two proteins (Gp3 and Gp4) of this phage. To our knowledge, Fnp?02 is the first phage described to infect Fusobacterium nucleatum and provides the base for future exploration of phages in the control of periodontal disease.

SUBMITTER: Machuca P

PROVIDER: S-EPMC2976222 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Isolation of a novel bacteriophage specific for the periodontal pathogen Fusobacterium nucleatum.

Machuca Pamela P Daille Leslie L Vinés Enrique E Berrocal Liliana L Bittner Mauricio M

Applied and environmental microbiology 20100917 21

Fusobacterium nucleatum is a periodontal pathogen that has been directly associated with the development and progression of periodontal disease, a widespread pathology that affects the support tissues of the tooth. We isolated a new bacteriophage (FnpΦ02) that specifically infects this bacterium. Transmission electron microscopy showed that the virion is composed of an icosahedral head and a segmented tail. The size of the phage genome was estimated to be approximately 59 kbp of double-stranded ...[more]

PMID: 20851973

Similar Datasets

Project description:BackgroundInfection with the periodontal pathogen Fusobacterium nucleatum (F. nucleatum) has been associated with gastric cancer. The present study is aimed at uncovering the putative biological mechanisms underlying effects of F. nucleatum-mediated neutrophil transcriptional deregulation in gastric cancer.Materials and methodsA gene expression dataset pertaining to F. nucleatum-infected human neutrophils was utilized to identify differentially expressed genes (DEGs) using the GEO2R tool. Candidate genes associated with gastric cancer were sourced from the "Candidate Cancer Gene Database" (CCGD). Overlapping genes among these were identified as link genes. Functional profiling of the link genes was performed using "g:Profiler" tool to identify enriched Gene Ontology (GO) terms, pathways, miRNAs, transcription factors, and human phenotype ontology terms. Protein-protein interaction (PPI) network was constructed for the link genes using the "STRING" tool, hub nodes were identified as key candidate genes, and functionally enriched terms were determined.ResultsThe gene expression dataset GEO20151 was downloaded, and 589 DEGs were identified through differential analysis. 886 candidate gastric cancer genes were identified in the CGGD database. Among these, 36 overlapping genes were identified as the link genes. Enriched GO terms included molecular function "enzyme building," biological process "protein folding,'" cellular components related to membrane-bound organelles, transcription factors ER71 and Sp1, miRNAs miR580 and miR155, and several human phenotype ontology terms including squamous epithelium of esophagus. The PPI network contained 36 nodes and 53 edges, where the top nodes included PH4 and CANX, and functional terms related to intracellular membrane trafficking were enriched.ConclusionF nucleatum-induced neutrophil transcriptional activation may be implicated in gastric cancer via several candidate genes including DNAJB1, EHD1, IER2, CANX, and PH4B. Functional analysis revealed membrane-bound organelle dysfunction, intracellular trafficking, transcription factors ER71 and Sp1, and miRNAs miR580 and miR155 as other candidate mechanisms, which should be investigated in experimental studies.

Dataset Information

Isolation of a novel bacteriophage specific for the periodontal pathogen Fusobacterium nucleatum.

Publications

Isolation of a novel bacteriophage specific for the periodontal pathogen Fusobacterium nucleatum.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets