Development of a real-time qPCR method for detection and enumeration of Mycobacterium spp. in surface water.
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ABSTRACT:
SUBMITTER: Radomski N
PROVIDER: S-EPMC2976254 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Publications
Development of a real-time qPCR method for detection and enumeration of Mycobacterium spp. in surface water.
Radomski Nicolas N Lucas Françoise S FS Moilleron Régis R Cambau Emmanuelle E Haenn Sophie S Moulin Laurent L
Applied and environmental microbiology 20100917 21
A real-time quantitative PCR method was developed for the detection and enumeration of Mycobacterium spp. from environmental samples and was compared to two other methods already described. The results showed that our method, targeting 16S rRNA, was more specific than the two previously published real-time quantitative PCR methods targeting another 16S rRNA locus and the hsp65 gene (100% versus 44% and 91%, respectively). ...[more]
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