Ontology highlight
ABSTRACT:
SUBMITTER: Bhartia R
PROVIDER: S-EPMC2976275 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Applied and environmental microbiology 20100903 21
We introduce a near-real-time optical imaging method that works via the detection of the intrinsic fluorescence of life forms upon excitation by deep-UV (DUV) illumination. A DUV (<250-nm) source enables the detection of microbes in their native state on natural materials, avoiding background autofluorescence and without the need for fluorescent dyes or tags. We demonstrate that DUV-laser-induced native fluorescence can detect bacteria on opaque surfaces at spatial scales ranging from tens of ce ...[more]