Ontology highlight
ABSTRACT:
SUBMITTER: Mouw KW
PROVIDER: S-EPMC2976518 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Journal of molecular biology 20100922 1
Serine recombinases promote specific DNA rearrangements by a cut-and-paste mechanism that involves cleavage of all four DNA strands at two sites recognized by the enzyme. Dissecting the order and timing of these cleavage events and the steps leading up to them is difficult because the cleavage reaction is readily reversible. Here, we describe assays using activated Sin mutants and a DNA substrate with a 3'-bridging phosphorothiolate modification that renders Sin-mediated DNA cleavage irreversibl ...[more]