Unknown

Dataset Information

0

Quantum-dot-decorated robust transductable bioluminescent nanocapsules.


ABSTRACT: Bioluminescence, due to its high sensitivity, has been exploited in various analytical and imaging applications. In this work, we report a highly stable, cell-transductable, and wavelength-tunable bioluminescence system achieved with an elegant and simple design. Using aqueous in situ polymerization on a bioluminescent enzyme anchored with polymerizable vinyl groups, we obtained nanosized core-shell nanocapsules with the enzyme as the core and a cross-linked thin polymer net as the shell. These nanocapsules possess greatly enhanced stability, retained bioactivity, and a readily engineered surface. In particular, by incorporating polymerizable amines in the polymerization, we endowed the nanocapsules with efficient cell-transduction and sufficient conjugation sites for follow-up modification. Following in situ polymerization, decorating the polymer shell with fluorescent quantum dots allowed us to access a continuous tunable wavelength, which extends the application of such bioluminescent nanocapsules, especially in deep tissue. In addition, the unique core-shell structure and adequate conjugation sites on surface enabled us to maximize the BRET efficiency by adjusting the QD/enzyme conjugation ratio.

SUBMITTER: Du J 

PROVIDER: S-EPMC2976844 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Quantum-dot-decorated robust transductable bioluminescent nanocapsules.

Du Juanjuan J   Yu Changming C   Pan Daocheng D   Li Jianmin J   Chen Wei W   Yan Ming M   Segura Tatiana T   Lu Yunfeng Y  

Journal of the American Chemical Society 20100901 37


Bioluminescence, due to its high sensitivity, has been exploited in various analytical and imaging applications. In this work, we report a highly stable, cell-transductable, and wavelength-tunable bioluminescence system achieved with an elegant and simple design. Using aqueous in situ polymerization on a bioluminescent enzyme anchored with polymerizable vinyl groups, we obtained nanosized core-shell nanocapsules with the enzyme as the core and a cross-linked thin polymer net as the shell. These  ...[more]

Similar Datasets

| S-EPMC11315915 | biostudies-literature
| S-EPMC3062676 | biostudies-literature
| S-EPMC6474140 | biostudies-literature
| S-EPMC7387828 | biostudies-literature
| S-EPMC3739287 | biostudies-literature
| S-EPMC3814187 | biostudies-literature
| S-EPMC6369982 | biostudies-literature
| S-EPMC8602655 | biostudies-literature
| S-EPMC5589847 | biostudies-literature
| S-EPMC5673898 | biostudies-literature