PCR with an expanded genetic alphabet.
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ABSTRACT:
SUBMITTER: Malyshev DA
PROVIDER: S-EPMC2978235 | biostudies-literature | 2009 Oct
REPOSITORIES: biostudies-literature
Publications
PCR with an expanded genetic alphabet.
Malyshev Denis A DA Seo Young Jun YJ Ordoukhanian Phillip P Romesberg Floyd E FE
Journal of the American Chemical Society 20091001 41
Expansion of the genetic alphabet with a third base pair would lay the foundation for a semisynthetic organism with an expanded genetic code and also have immediate in vitro applications. Previously, the unnatural base pairs formed between d5SICS and either dNaM or dMMO2 were shown to be well-replicated by DNA polymerases under steady-state conditions and also transcribed by T7 RNA polymerase efficiently in either direction. We now demonstrate that DNA containing either the d5SICS-dNaM or d5SICS ...[more]
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