CyDye immunoblotting for proteomics: co-detection of specific immunoreactive and total protein profiles.
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ABSTRACT:
SUBMITTER: Donoghue PM
PROVIDER: S-EPMC2978322 | biostudies-literature | 2006 Dec
REPOSITORIES: biostudies-literature
Publications
CyDye immunoblotting for proteomics: co-detection of specific immunoreactive and total protein profiles.
Donoghue Pamela M PM McManus Ciara A CA O'Donoghue Niaobh M NM Pennington Stephen R SR Dunn Michael J MJ
Proteomics 20061201 24
The development of ECL-Plex CyDye-conjugated secondary antibodies allows the advancement of conventional Western blotting, opening up possibilities for highly sensitive and quantitative protein confirmation and identification. We report a novel proteomic method to simultaneously visualise the total protein profile as well as the specific immunodetection of an individual protein species by combining cyanine CyDye pre-labelled proteins and antibody immunoblotting. This technique proposes to revolu ...[more]
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