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CyDye immunoblotting for proteomics: co-detection of specific immunoreactive and total protein profiles.

ABSTRACT: The development of ECL-Plex CyDye-conjugated secondary antibodies allows the advancement of conventional Western blotting, opening up possibilities for highly sensitive and quantitative protein confirmation and identification. We report a novel proteomic method to simultaneously visualise the total protein profile as well as the specific immunodetection of an individual protein species by combining cyanine CyDye pre-labelled proteins and antibody immunoblotting. This technique proposes to revolutionise both 2-D immunoprobing and protein confirmation following MS analysis.

SUBMITTER: Donoghue PM

PROVIDER: S-EPMC2978322 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature

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CyDye immunoblotting for proteomics: co-detection of specific immunoreactive and total protein profiles.

Donoghue Pamela M PM McManus Ciara A CA O'Donoghue Niaobh M NM Pennington Stephen R SR Dunn Michael J MJ

Proteomics 20061201 24

The development of ECL-Plex CyDye-conjugated secondary antibodies allows the advancement of conventional Western blotting, opening up possibilities for highly sensitive and quantitative protein confirmation and identification. We report a novel proteomic method to simultaneously visualise the total protein profile as well as the specific immunodetection of an individual protein species by combining cyanine CyDye pre-labelled proteins and antibody immunoblotting. This technique proposes to revolu ...[more]

PMID: 17111436

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Dataset Information

CyDye immunoblotting for proteomics: co-detection of specific immunoreactive and total protein profiles.

Publications

CyDye immunoblotting for proteomics: co-detection of specific immunoreactive and total protein profiles.

Similar Datasets

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