Ontology highlight
ABSTRACT:
SUBMITTER: Baronio R
PROVIDER: S-EPMC2978351 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Baronio Roberta R Danziger Samuel A SA Hall Linda V LV Salmon Kirsty K Hatfield G Wesley GW Lathrop Richard H RH Kaiser Peter P
Nucleic acids research 20100625 20
In vitro scanning mutagenesis strategies are valuable tools to identify critical residues in proteins and to generate proteins with modified properties. We describe the fast and simple All-Codon Scanning (ACS) strategy that creates a defined gene library wherein each individual codon within a specific target region is changed into all possible codons with only a single codon change per mutagenesis product. ACS is based on a multiplexed overlapping mutagenesis primer design that saturates only th ...[more]