Ontology highlight
ABSTRACT:
SUBMITTER: Uejima T
PROVIDER: S-EPMC2978598 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Uejima Tamami T Ihara Kentaro K Goh Tatsuaki T Ito Emi E Sunada Mariko M Ueda Takashi T Nakano Akihiko A Wakatsuki Soichi S
The Journal of biological chemistry 20100910 47
Many GTPases regulate intracellular transport and signaling in eukaryotes. Guanine nucleotide exchange factors (GEFs) activate GTPases by catalyzing the exchange of their GDP for GTP. Here we present crystallographic and biochemical studies of a GEF reaction with four crystal structures of Arabidopsis thaliana ARA7, a plant homolog of Rab5 GTPase, in complex with its GEF, VPS9a, in the nucleotide-free and GDP-bound forms, as well as a complex with aminophosphonic acid-guanylate ester and ARA7·VP ...[more]