Alta-Cyclic: a self-optimizing base caller for next-generation sequencing.
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ABSTRACT:
SUBMITTER: Erlich Y
PROVIDER: S-EPMC2978646 | biostudies-literature | 2008 Aug
REPOSITORIES: biostudies-literature
Publications
Alta-Cyclic: a self-optimizing base caller for next-generation sequencing.
Erlich Yaniv Y Mitra Partha P PP delaBastide Melissa M McCombie W Richard WR Hannon Gregory J GJ
Nature methods 20080706 8
Next-generation sequencing is limited to short read lengths and by high error rates. We systematically analyzed sources of noise in the Illumina Genome Analyzer that contribute to these high error rates and developed a base caller, Alta-Cyclic, that uses machine learning to compensate for noise factors. Alta-Cyclic substantially improved the number of accurate reads for sequencing runs up to 78 bases and reduced systematic biases, facilitating confident identification of sequence variants. ...[more]
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