Unknown

Dataset Information

0

Ion accumulation in a protein nanocage: finding noisy temporal sequences using a genetic algorithm.


ABSTRACT: Many pathogenic bacteria are able to survive attack by the host's immune system because of antioxidant systems that mitigate the effects of reactive oxygen species. Dps is a hollow 12-subunit protein nanocage that prevents oxidative damage by oxidizing and sequestering intracellular Fe(2+); the resulting Fe(3+) forms an iron oxyhydroxide nanoparticle in the cage interior. Charged sites on the protein nanocage create an electrostatic gradient that guides ions through well-defined pores that connect the cage interior with the surrounding solution and toward nucleation sites on the cage interior. In this study, we use all-atom molecular dynamics to simulate the motion of simple cations into the dodecameric cage formed by the Dps protein from Listeria monocytogenes. Ion trajectories are analyzed by using a novel, to our knowledge, genetic algorithm to determine the temporal sequence of ion-protein interactions. Ions enter Dps through well-defined pores at the ferritinlike C(3) axes, with negatively-charged residues on the outside of the cage forming a fairly well-defined entrance pathway. This method of trajectory analysis may be broadly applicable in situations where the spatial localization of ions or other small molecules is electrostatically driven by a biomolecule.

SUBMITTER: Jolley CC 

PROVIDER: S-EPMC2980703 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Ion accumulation in a protein nanocage: finding noisy temporal sequences using a genetic algorithm.

Jolley Craig C CC   Douglas Trevor T  

Biophysical journal 20101101 10


Many pathogenic bacteria are able to survive attack by the host's immune system because of antioxidant systems that mitigate the effects of reactive oxygen species. Dps is a hollow 12-subunit protein nanocage that prevents oxidative damage by oxidizing and sequestering intracellular Fe(2+); the resulting Fe(3+) forms an iron oxyhydroxide nanoparticle in the cage interior. Charged sites on the protein nanocage create an electrostatic gradient that guides ions through well-defined pores that conne  ...[more]

Similar Datasets

| S-EPMC2978673 | biostudies-literature
| S-EPMC1803799 | biostudies-literature
| S-EPMC434454 | biostudies-literature
| S-EPMC5655831 | biostudies-other
| S-EPMC8476132 | biostudies-literature
| S-EPMC3339931 | biostudies-literature
| S-EPMC6981237 | biostudies-literature
| S-EPMC7068855 | biostudies-literature
| S-EPMC1963494 | biostudies-literature
| S-EPMC4447411 | biostudies-literature