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High-redundancy draft sequencing of 15 clinical and environmental Burkholderia strains.

ABSTRACT: The Gram-negative Burkholderia genus includes several species of intracellular bacterial pathogens that pose substantial risk to humans. In this study, we have generated draft genome sequences of 15 strains of B. oklahomensis, B. pseudomallei, B. thailandensis, and B. ubonensis to an average sequence read coverage of 25- to 40-fold.

SUBMITTER: Mukhopadhyay S

PROVIDER: S-EPMC2981208 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature

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High-redundancy draft sequencing of 15 clinical and environmental Burkholderia strains.

Mukhopadhyay Sanghamitra S Thomason Maureen K MK Lentz Shannon S Nolan Nichole N Willner Kristin K Gee Jay E JE Glass Mindy B MB Inglis Timothy J J TJ Merritt Adam A Levy Avram A Sozhamannan Shanmuga S Mateczun Al A Read Timothy D TD

Journal of bacteriology 20100924 23

The Gram-negative Burkholderia genus includes several species of intracellular bacterial pathogens that pose substantial risk to humans. In this study, we have generated draft genome sequences of 15 strains of B. oklahomensis, B. pseudomallei, B. thailandensis, and B. ubonensis to an average sequence read coverage of 25- to 40-fold. ...[more]

PMID: 20870763

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Project description:Background Burkholderia pseudomallei (B. pseudomallei) is a highly infectious agent and causes melioidosis, in both humans and animals, which is endemic in Southeast Asia and Northern Australia. Objectives This study aims to determine the molecular epidemiology, resistant determinants, and genomic diversity of the clinical isolates of B. pseudomallei to further elucidate the phylogenetic and evolutionary relationship of the strains with those in other endemic regions. Methods In this study, we obtained eight clinical B. pseudomallei isolates from Guangdong province from 2018 to 2019. All the isolates were sequenced using the Illumina NovaSeq platform. The draft genomes of B. pseudomallei were further used to find antibiotic-resistant genes (ARGs), virulence factors, and gene mutations. Multilocus sequence typing (MLST) and single nucleotide polymorphism (SNP) analysis were performed to characterize the diversity and epidemiology of the strains. Results All isolates were susceptible to antibiotics commonly used for melioidosis treatment. Class D beta-lactamases genes OXA-57 and OXA-59, as well as various mutation factors such as amrA, amrB, omp38, gyrA, and ceoB were identified. MLST analysis of the B. pseudomallei strains identified eight different sequence types (STs): ST1774, ST1775, ST271, ST562, ST46, ST830, ST1325, and ST10. Phylogenetic analysis found that the strains used in this study showed high genetic diversity. We also report 165 virulence factors among B. pseudomallei strains responsible for different neurological disorders, pneumonia, skin lesions, and abscesses. All strains recovered in this study were susceptible to commonly used antibiotics. However, high genetic diversity exists among the isolates. The surveillance, diagnosis, and clinical features of melioidosis varied in different geographical locations. These regional differences in the clinical manifestations have implications for the practical management of the disease. Conclusion The present study reports the identification of different mutation and virulence factors among B. pseudomallei strains responsible for different neurological disorders, pneumonia, skin lesions, and abscesses.

Dataset Information

High-redundancy draft sequencing of 15 clinical and environmental Burkholderia strains.

Publications

High-redundancy draft sequencing of 15 clinical and environmental Burkholderia strains.

Similar Datasets

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