Photochemical regulation of restriction endonuclease activity.

ABSTRACT: Removal by the light: The photochemical regulation of restriction endonucleases, which are important enzymes in molecular biology, has been investigated. Photolabile protecting groups have been installed on DNA substrates and have been demonstrated to inhibit restriction endonuclease activity until removed by UV light irradiation. Interestingly, these groups do not appear to dramatically affect initial binding of the enzyme to the DNA substrate, but rather prevent recognition of the specific cleavage site.

SUBMITTER: Young DD 

PROVIDER: S-EPMC2983471 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature