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Gene expression analysis of cell death induction by taurolidine in different malignant cell lines.

ABSTRACT: BACKGROUND: The anti-infective agent Taurolidine (TRD) has been shown to have cell death inducing properties, but the mechanism of its action is largely unknown. The aim of this study was to identify potential common target genes modulated at the transcriptional level following TRD treatment in tumour cell lines originating from different cancer types. METHODS: Five different malignant cell lines (HT29, Chang Liver, HT1080, AsPC-1 and BxPC-3) were incubated with TRD (100 ?M, 250 ?M and 1000 ?M). Proliferation after 8 h and cell viability after 24 h were analyzed by BrdU assay and FACS analysis, respectively. Gene expression analyses were carried out using the Agilent-microarray platform to identify genes which displayed conjoint regulation following the addition of TRD in all cell lines. Candidate genes were subjected to Ingenuity Pathways Analysis and selected genes were validated by qRT-PCR and Western Blot. RESULTS: TRD 250 ?M caused a significant inhibition of proliferation as well as apoptotic cell death in all cell lines. Among cell death associated genes with the strongest regulation in gene expression, we identified pro-apoptotic transcription factors (EGR1, ATF3) as well as genes involved in the ER stress response (PPP1R15A), in ubiquitination (TRAF6) and mitochondrial apoptotic pathways (PMAIP1). CONCLUSIONS: This is the first conjoint analysis of potential target genes of TRD which was performed simultaneously in different malignant cell lines. The results indicate that TRD might be involved in different signal transduction pathways leading to apoptosis.

SUBMITTER: Chromik AM

PROVIDER: S-EPMC2988031 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature

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Gene expression analysis of cell death induction by taurolidine in different malignant cell lines.

Chromik Ansgar M AM Hahn Stephan A SA Daigeler Adrien A Flier Annegret A Bulut Daniel D May Christina C Harati Kamran K Roschinsky Jan J Sülberg Dominique D Weyhe Dirk D Mittelkötter Ulrich U Uhl Waldemar W

BMC cancer 20101030

<h4>Background</h4>The anti-infective agent Taurolidine (TRD) has been shown to have cell death inducing properties, but the mechanism of its action is largely unknown. The aim of this study was to identify potential common target genes modulated at the transcriptional level following TRD treatment in tumour cell lines originating from different cancer types.<h4>Methods</h4>Five different malignant cell lines (HT29, Chang Liver, HT1080, AsPC-1 and BxPC-3) were incubated with TRD (100 μM, 250 μM ...[more]

PMID: 21034493

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Dataset Information

Gene expression analysis of cell death induction by taurolidine in different malignant cell lines.

Publications

Gene expression analysis of cell death induction by taurolidine in different malignant cell lines.

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