Discrete structure of an RNA folding intermediate revealed by cryo-electron microscopy.
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ABSTRACT:
SUBMITTER: Baird NJ
PROVIDER: S-EPMC2988076 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Publications
Discrete structure of an RNA folding intermediate revealed by cryo-electron microscopy.
Baird Nathan J NJ Ludtke Steven J SJ Khant Htet H Chiu Wah W Pan Tao T Sosnick Tobin R TR
Journal of the American Chemical Society 20101101 46
RNA folding occurs via a series of transitions between metastable intermediate states. It is unknown whether folding intermediates are discrete structures folding along defined pathways or heterogeneous ensembles folding along broad landscapes. We use cryo-electron microscopy and single-particle image reconstruction to determine the structure of the major folding intermediate of the specificity domain of a ribonuclease P ribozyme. Our results support the existence of a discrete conformation for ...[more]
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