Ontology highlight
ABSTRACT:
SUBMITTER: Gauthier DJ
PROVIDER: S-EPMC2989539 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
Gauthier Daniel J DJ Sobota Jacqueline A JA Ferraro Francesco F Mains Richard E RE Lazure Claude C
Proteomics 20080901 18
The field of organellar proteomics has emerged as an attempt to minimize the complexity of the proteomics data obtained from whole cell and tissue extracts while maximizing the resolution on the protein composition of a single subcellular compartment. Standard methods involve lengthy density-based gradient and/or immunoaffinity purification steps followed by extraction, 1-DE or 2-DE, gel staining, in-gel tryptic digestion, and protein identification by MS. In this paper, we present an alternate ...[more]