Ontology highlight
ABSTRACT:
SUBMITTER: Wevers D
PROVIDER: S-EPMC2989969 | biostudies-literature | 2010
REPOSITORIES: biostudies-literature
Wevers Diana D Leendertz Fabian H FH Scuda Nelly N Boesch Christophe C Robbins Martha M MM Head Josephine J Ludwig Carsten C Kühn Joachim J Ehlers Bernhard B
Virology journal 20101105
Adenoviruses (AdV) broadly infect vertebrate hosts including a variety of primates. We identified a novel AdV in the feces of captive gorillas by isolation in cell culture, electron microscopy and PCR. From the supernatants of infected cultures we amplified DNA polymerase (DPOL), preterminal protein (pTP) and hexon gene sequences with generic pan primate AdV PCR assays. The sequences in-between were amplified by long-distance PCRs of 2-10 kb length, resulting in a final sequence of 15.6 kb. Phyl ...[more]