Ontology highlight
ABSTRACT:
SUBMITTER: Hodges E
PROVIDER: S-EPMC2990409 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Hodges Emily E Rooks Michelle M Xuan Zhenyu Z Bhattacharjee Arindam A Benjamin Gordon D D Brizuela Leonardo L Richard McCombie W W Hannon Gregory J GJ
Nature protocols 20090528 6
Complementary techniques that deepen information content and minimize reagent costs are required to realize the full potential of massively parallel sequencing. Here, we describe a resequencing approach that directs focus to genomic regions of high interest by combining hybridization-based purification of multi-megabase regions with sequencing on the Illumina Genome Analyzer (GA). The capture matrix is created by a microarray on which probes can be programmed as desired to target any non-repeat ...[more]