Dataset Information

Evidence of prokineticin dysregulation in fallopian tube from women with ectopic pregnancy.

ABSTRACT:

Objective

To demonstrate expression and regulation of prokineticins (PROKs) and their receptors (PROKRs) in fallopian tube (FT) from women who are not pregnant and women with ectopic pregnancy (EP).

Design

Tissue analysis.

Setting

Large United Kingdom teaching hospital.

Patient(s)

Women undergoing hysterectomy for benign gynecological conditions (n = 15) and surgery for EP (n = 16).

Intervention(s)

Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry were used to determine FT PROK/PROKR messenger RNA (mRNA) expression and protein localization, respectively. The PROK/PROKR levels were measured in tubal explant cultures stimulated with estrogen (E) and progestogen.

Main outcome measure(s)

Differential expression of PROK and PROKR.

Result(s)

The FT PROK2 and PROKR1 mRNA levels were up-regulated during the P-dominant midluteal phase of the menstrual cycle. Increased PROKR1 expression was observed in tubal explant cultures treated with medroxy-progesterone acetate (MPA). The PROK and PROKR proteins were localized to the epithelium and smooth muscle layers of the FT. The PROKR1 and PROKR2 mRNA levels were lower in FT from women with EP compared with nonpregnant FT from the midluteal phase.

Conclusion(s)

These data suggest a potential role for PROKs in FT function. The PROKs are known to affect smooth muscle contraction in the gut. Dysregulated PROK expression in FT could affect FT smooth muscle contractility and embryo-tubal transport, providing a potential cause for EP.

SUBMITTER: Shaw JL

PROVIDER: S-EPMC2990462 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Evidence of prokineticin dysregulation in fallopian tube from women with ectopic pregnancy.

Shaw Julie L V JL Denison Fiona C FC Evans Jemma J Durno Kimberley K Williams Alistair R AR Entrican Gary G Critchley Hilary O D HO Jabbour Henry N HN Horne Andrew W AW

Fertility and sterility 20100104 5

<h4>Objective</h4>To demonstrate expression and regulation of prokineticins (PROKs) and their receptors (PROKRs) in fallopian tube (FT) from women who are not pregnant and women with ectopic pregnancy (EP).<h4>Design</h4>Tissue analysis.<h4>Setting</h4>Large United Kingdom teaching hospital.<h4>Patient(s)</h4>Women undergoing hysterectomy for benign gynecological conditions (n = 15) and surgery for EP (n = 16).<h4>Intervention(s)</h4>Quantitative reverse transcriptase-polymerase chain reaction ( ...[more]

PMID: 20047737

Similar Datasets

Project description:BackgroundEmbryo retention in the Fallopian tube (FT) is thought to lead to ectopic pregnancy (EP), a considerable cause of morbidity. In mice, genetic/pharmacological silencing of cannabinoid receptor Cnr1, encoding CB1, causes retention of embryos in the oviduct. The role of the endocannabinoids in tubal implantation in humans is not known.Methods and findingsTimed FT biopsies (n = 18) were collected from women undergoing gynecological procedures for benign conditions. Endometrial biopsies and whole blood were collected from women undergoing surgery for EP (n = 11); management of miscarriage (n = 6), and termination of pregnancy (n = 8). Using RT-PCR and immunohistochemistry, CB1 mRNA and protein expression levels/patterns were examined in FT and endometrial biopsies. The distribution of two polymorphisms of CNR1 was examined by TaqMan analysis of genomic DNA from the whole blood samples. In normal FT, CB1 mRNA was higher in luteal compared to follicular-phase (p<0.05). CB1 protein was located in smooth muscle of the wall and of endothelial vessels, and luminal epithelium of FT. In FT from women with EP, CB1 mRNA expression was low. CB1 mRNA expression was also significantly lower (p<0.05) in endometrium of women with EP compared to intrauterine pregnancies (IUP). Although of 1359G/A (rs1049353) polymorphisms of CNR1 gene suggests differential distribution of genotypes between the small, available cohorts of women with EP and those with IUP, results were not statistically significant.ConclusionsCB1 mRNA shows temporal variation in expression in human FT, likely regulated by progesterone. CB1 mRNA is expressed in low levels in both the FT and endometrium of women with EP. We propose that aberrant endocannabinoid-signaling in human FT leads to EP. Furthermore, our finding of reduced mRNA expression along with a possible association between polymorphism genotypes of the CNR1 gene and EP, suggests a possible genetic predisposition to EP that warrants replication in a larger sample pool.

Dataset Information

Evidence of prokineticin dysregulation in fallopian tube from women with ectopic pregnancy.

Objective

Design

Setting

Patient(s)

Intervention(s)

Main outcome measure(s)

Result(s)

Conclusion(s)

Publications

Evidence of prokineticin dysregulation in fallopian tube from women with ectopic pregnancy.

Similar Datasets

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