Ontology highlight
ABSTRACT:
SUBMITTER: Sengupta J
PROVIDER: S-EPMC2990977 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
Sengupta Jayati J Nilsson Jakob J Gursky Richard R Kjeldgaard Morten M Nissen Poul P Frank Joachim J
Journal of molecular biology 20080711 1
In an attempt to understand ribosome-induced GTP hydrolysis on eEF2, we determined a 12.6-A cryo-electron microscopy reconstruction of the eEF2-bound 80S ribosome in the presence of aluminum tetrafluoride and GDP, with aluminum tetrafluoride mimicking the gamma-phosphate during hydrolysis. This is the first visualization of a structure representing a transition-state complex on the ribosome. Tight interactions are observed between the factor's G domain and the large ribosomal subunit, as well as ...[more]