Ontology highlight
ABSTRACT:
SUBMITTER: Kovarik ML
PROVIDER: S-EPMC2992552 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Kovarik Michelle L ML Brown Pamela J B PJ Kysela David T DT Berne Cécile C Kinsella Anna C AC Brun Yves V YV Jacobson Stephen C SC
Analytical chemistry 20101020 22
Motile bacteria bias the random walk of their motion in response to chemical gradients by the process termed chemotaxis, which allows cells to accumulate in favorable environments and disperse from less favorable ones. In this work, we describe a simple microchannel-nanopore device that establishes a stable chemical gradient for chemotaxis assays in ≤1 min. Chemoattractant is dispensed by diffusion through 10 nm diameter pores at the intersection of two microchannels. This design requires no ext ...[more]