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Release of content through mechano-sensitive gates in pressurized liposomes.

ABSTRACT: Mechano-sensitive channels are ubiquitous membrane proteins that activate in response to increasing tension in the lipid membrane. They facilitate a sudden, nonselective release of solutes and water that safeguards the integrity of the cell in hypo- or hyper-osmotic shock conditions. We have simulated the rapid release of content from a pressurized liposome through a particular mechano-sensitive protein channel, MscL, embedded in the liposomal membrane. We show that a single channel is able to relax the liposome, stressed to the point of bursting, in a matter of microseconds. We map the full activation-deactivation cycle of MscL in near-atomic detail and are able to quantify the rapid decrease in liposomal stress as a result of channel activation. This provides a computational tool that opens the way to contribute to the rational design of functional nano-containers.

SUBMITTER: Louhivuori M

PROVIDER: S-EPMC2993341 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

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Release of content through mechano-sensitive gates in pressurized liposomes.

Louhivuori Martti M Risselada H Jelger HJ van der Giessen Erik E Marrink Siewert J SJ

Proceedings of the National Academy of Sciences of the United States of America 20101101 46

Mechano-sensitive channels are ubiquitous membrane proteins that activate in response to increasing tension in the lipid membrane. They facilitate a sudden, nonselective release of solutes and water that safeguards the integrity of the cell in hypo- or hyper-osmotic shock conditions. We have simulated the rapid release of content from a pressurized liposome through a particular mechano-sensitive protein channel, MscL, embedded in the liposomal membrane. We show that a single channel is able to r ...[more]

PMID: 21041677

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