Ontology highlight
ABSTRACT:
SUBMITTER: Arai M
PROVIDER: S-EPMC2993637 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
FEBS letters 20101020 22
Physical interaction between the transactivation domain (TAD) of the mixed-lineage leukemia protein (MLL) and the KIX domain of the cyclic-AMP response element binding protein (CREB) binding protein (CBP) is necessary for MLL-mediated transcriptional activation. We show by alanine-scanning mutagenesis that hydrophobic surface residues of KIX, especially L628, are energetically important for binding the MLL TAD. NMR studies of the KIX-L628A mutant suggest that L628 plays a crucial role in conform ...[more]