Oriented insertion of phi29 N-hexahistidine-tagged gp10 connector protein assemblies into C20BAS bolalipid membrane vesicles.
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ABSTRACT:
SUBMITTER: Hyun SH
PROVIDER: S-EPMC2995011 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Publications
Oriented insertion of phi29 N-hexahistidine-tagged gp10 connector protein assemblies into C20BAS bolalipid membrane vesicles.
Hyun Seok-Hee SH Kim Hee-kwon HK Kim Jong-Mok JM Thompson David H DH
Journal of the American Chemical Society 20100817 48
Ni(2+):NTA-PEG600-grafted glass surfaces are capable of immobilizing N-his6 gp10 connector protein assemblies from the phi29 DNA packaging motor and mediating their transplantation into bolalipid vesicles whose membrane thickness is compatible with the hydrophobic domain of the gp10 assemblies. ...[more]
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