Ontology highlight
ABSTRACT:
SUBMITTER: Guedin A
PROVIDER: S-EPMC2995061 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Guédin Aurore A Gros Julien J Alberti Patrizia P Mergny Jean-Louis JL
Nucleic acids research 20100726 21
We compared here 80 different sequences containing four tracts of three guanines with loops of variable length (between 1 and 15 bases for unmodified sequences, up to 30 for fluorescently labeled oligonucleotides). All sequences were capable of forming stable quadruplexes, with T(m) above physiological temperature in most cases. Unsurprisingly, the melting temperature was systematically lower in sodium than in potassium but the difference between both ionic conditions varied between 1 and >39°C ...[more]