The overmethylated genes in Helicobacter pylori-infected gastric mucosa are demethylated in gastric cancers.

ABSTRACT:

Background

The transitional-CpG sites between weakly methylated genes and densely methylated retroelements are overmethylated in the gastric mucosa infected with Helicobacter pylori (H. pylori) and they are undermethylated in the gastric cancers depending on the level of loss of heterozygosity (LOH) events. This study delineated the transitional-CpG methylation patterns of CpG-island-containing and -lacking genes in view of the retroelements.

Methods

The transitional-CpG sites of eight CpG-island-containing genes and six CpG-island-lacking genes were semi-quantitatively examined by performing radioisotope-labelling methylation-specific PCR under stringent conditions. The level of LOH in the gastric cancers was estimated using the 40 microsatellite markers on eight cancer-ass

SUBMITTER: Hong SJ 

PROVIDER: S-EPMC2995475 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature