T regulatory cells maintain intestinal homeostasis by suppressing ?? T cells.

ABSTRACT: Immune tolerance against enteric commensal bacteria is important for preventing intestinal inflammation. Deletion of phosphoinositide-dependent protein kinase 1 (Pdk1) in T cells via Cd4-Cre induced chronic inflammation of the intestine despite the importance of PDK1 in T cell activation. Analysis of colonic intraepithelial lymphocytes of PDK1-deficient mice revealed markedly increased CD8?(+) T cell receptor (TCR)??(+) T cells, including an interleukin-17 (IL-17)-expressing population. TCR??(+) T cells were responsible for the inflammatory colitis as shown by the fact that deletion of Tcrd abolished spontaneous colitis in the PDK1-deficient mice. This dysregulation of intestinal TCR??(+) T cells was attributable to a reduction in the number and functional capacity of PDK1-deficient T regulatory (Treg) cells. Adoptive transfer of wild-type Treg cells abrogated the spontaneous activation and proliferation of intestinal TCR??(+) T cells observed in PDK1-deficient mice and prevented the development of colitis. Therefore, suppression of intestinal TCR??(+) T cells by Treg cells maintains enteric immune tolerance.

SUBMITTER: Park SG 

PROVIDER: S-EPMC2996054 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature