Ontology highlight
ABSTRACT:
SUBMITTER: Knowles MK
PROVIDER: S-EPMC2996426 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Knowles M K MK Barg S S Wan L L Midorikawa M M Chen X X Almers Wolfhard W
Proceedings of the National Academy of Sciences of the United States of America 20101112 48
Before secretory vesicles undergo exocytosis, they must recruit the proteins syntaxin-1 and synaptosomal associated protein 25 (SNAP-25) in the plasma membrane. GFP-labeled versions of both proteins cluster at sites where secretory granules have docked. Single-particle tracking shows that minority populations of both molecules are strongly hindered in their mobility, consistent with their confinement in nanodomains. We measured the fluorescence of granule-associated clusters, the fluorescence of ...[more]