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Bulk segregation mapping of mutations in closely related strains of mice.

ABSTRACT: Phenovariance may be obscured when genetic mapping is performed using highly divergent strains, and closely similar strains are preferred if adequate marker density can be established. We sequenced the C57BL/10J mouse genome using the Applied Biosystems SOLiD platform and here describe a genome-wide panel of informative markers that permits the mapping of mutations induced on the closely related C57BL/6J background by outcrossing to C57BL/10J, and backcrossing or intercrossing. The panel consists of 127 single nucleotide polymorphisms validated by capillary sequencing: 124 spaced at ?20-Mb intervals across the 19 autosomes, and three markers on the X chromosome. We determined the genetic relationship between four C57BL-derived substrains and used the panel to map two N-ethyl-N-nitrosourea (ENU)-induced mutations responsible for visible phenotypes in C57BL/6J mice through bulk segregation analysis. Capillary sequencing, with computation of relative chromatogram peak heights, was used to determine the proportion of alleles from each strain at each marker.

SUBMITTER: Xia Y

PROVIDER: S-EPMC2998299 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature

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Bulk segregation mapping of mutations in closely related strains of mice.

Xia Yu Y Won Sungyong S Du Xin X Lin Pei P Ross Charles C La Vine Diantha D Wiltshire Sean S Leiva Gabriel G Vidal Silvia M SM Whittle Belinda B Goodnow Christopher C CC Koziol James J Moresco Eva Marie Y EM Beutler Bruce B

Genetics 20101005 4

Phenovariance may be obscured when genetic mapping is performed using highly divergent strains, and closely similar strains are preferred if adequate marker density can be established. We sequenced the C57BL/10J mouse genome using the Applied Biosystems SOLiD platform and here describe a genome-wide panel of informative markers that permits the mapping of mutations induced on the closely related C57BL/6J background by outcrossing to C57BL/10J, and backcrossing or intercrossing. The panel consist ...[more]

PMID: 20923982

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Bulk segregation mapping of mutations in closely related strains of mice.

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Bulk segregation mapping of mutations in closely related strains of mice.

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