Ontology highlight
ABSTRACT:
SUBMITTER: Gabrielsen M
PROVIDER: S-EPMC2998365 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Gabrielsen Mads M Zetterström Caroline E CE Wang Dai D Beckham Katherine S H KS Elofsson Mikael M Isaacs Neil W NW Roe Andrew J AJ
Acta crystallographica. Section F, Structural biology and crystallization communications 20101125 Pt 12
Thiol peroxidase is an atypical 2-Cys peroxiredoxin that reduces alkyl hydroperoxides. Wild-type and C61S mutant protein have been recombinantly expressed in Escherichia coli and purified using nickel-affinity chromatography. Initial crystallization trials yielded three crystal forms in three different space groups (P2(1), P6(4) and P2(1)2(1)2(1)) both in the presence and the absence of DTT. ...[more]