Ontology highlight
ABSTRACT:
SUBMITTER: Park CK
PROVIDER: S-EPMC2998434 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Park Chad K CK Joshi Hemant K HK Agrawal Alka A Ghare M Imran MI Little Elizabeth J EJ Dunten Pete W PW Bitinaite Jurate J Horton Nancy C NC
PLoS biology 20101207 12
SgrAI is a type IIF restriction endonuclease that cuts an unusually long recognition sequence and exhibits allosteric self-modulation of cleavage activity and sequence specificity. Previous studies have shown that DNA bound dimers of SgrAI oligomerize into an activated form with higher DNA cleavage rates, although previously determined crystal structures of SgrAI bound to DNA show only the DNA bound dimer. A new crystal structure of the type II restriction endonuclease SgrAI bound to DNA and Ca( ...[more]